DNA testing is analytically more sensitive than culture, especially in patients with encephalitis or meningitis. DNA testing may be useful in diagnosis of infection in neonates. Neonates who have been exposed to HSV can develop disseminated infection and encephalitis. Encephalitis is usually due to HSV I whereas meningitis is usually due to HSV II. DNA testing provides reliable means to define the Type.
Methodology: Real Time- Polymerase Chain Reaction (PCR)
Specimen Requirements: Submit 1.0 mL of cerebrospinal fluid (minimum of 0.3 mL) in a sterile screw-capped container supplied by Meadville Medical Center Laboratory. Send specimen frozen in plastic vial.
ACCEPTABLE SPECIMEN SOURCES: (1 mL; min of 0.3 mL)
Serum (red-top or SST)
Plasma (lavender-top EDTA)
Body Fluids (Pleural, Pericardial, Amniotic, Vitreous)
Swab (M4 viral transport medium)
Analytical Time: 2 days
Days Set Up: Monday through Sunday
87529 – x2